RNA detection for genetic markers of osteoblasts in juvenile animals assessed using RNAscope <i>in situ</i> hybridization on paraffin section.

<p>(A-F) Sequential sections (4 μm) of the interfrontal suture collected from juvenile zebrafish at age of 6 wpf. The expression of individual genes is visualized in red, counterstained with haematoxylin for nuclei in purple; black arrows examples of positive expression, all green arrows indicate negative for expression cells. (A) The expression of <i>col1a1a</i> is present in all cells. (B) The green arrow indicates cells that are negative for <i>col10a1a</i> expression in the mid-suture domain. (C) Expression of <i>bglap</i> weaker at the tips of frontal bones as indicated by blue arrow. (D) Expression of <i>sp7</i> at osteogenic fronts (blue arrow) and along the frontal bone (red arrow), (E) <i>runx2b</i> at the osteogenic front (blue arrow) and along the bone (black arrow). (F) <i>fgfr3</i> expression observed in osteogenic front (blue arrow) and along the frontal bones (black and purple arrows). The scale bar represents 20 μm.