Detectability of low frequency mutations in study cohort II.

<p>Blood from healthy donors (n = 8) was spiked with synthetic double-stranded mutant DNA fragments of different allele frequencies in a background of 60,000 genome equivalents of fragmented human genomic wild-type DNA and subsequently stored at RT. DNA was extracted and analyzed by BEAMing after the indicated storage time. Mean and standard deviation of the detected mutant fraction is shown. (A) <i>PIK3CA</i> c.3140A>G spike (0.1%), (B) <i>EGFR</i> c.2369C>T spike (0.5%), (C) <i>KRAS</i> c.34G>A spike (1%).