Protein expression by Western blotting images and quantification of signal transduction pathways in myocardium after cardiac arrest and resuscitation.

<p>(a) Akt (b) ERK42/44 in total lysates of myocardium at 2 h after cardiac arrest and resuscitation. Phosphorylation of Akt and ERK 42/44 was greater in the urocortin-treated group (*: P<0.05 for both). (c) & (d) Western blot showing STAT-3 phosphorylation with STAT-3 p-Tyr705 and STAT-3 p-Ser727 in the myocardium with and without urocortin treatment, respectively. After normalization to total amounts of STAT-3 protein, quantification of STAT-3 expression by Western blotting showed that more phosphorylated STAT-3 (Tyr 705) as well as STAT-3 (Ser 727) was expressed with urocortin treatment (n = 6 in each group, *: P < 0.05).