Embryonic lethal phenotypes in <i>C</i>. <i>elegans</i> and PaDAW1 after feeding <i>E</i>. <i>coli</i> expressing dsRNA.

<p>(A) Larval hatching of <i>gfp(RNAi)</i>-treated, <i>Pd-rps-2(RNAi)</i>-treated and <i>Pd-dhc(RNAi)</i>-treated PaDAW1 (left y-axes) and <i>C</i>. <i>elegans</i> (right y-axes) 48 h after removal of adults from the plates. Each value represents the mean±s.d. of 6–18 biological replicates (three nematodes per biological replicate). For PaDAW1 the experiment was replicated three times (six biological replicates per experiment) and the values represent the mean±s.d. of all 18 biological replicates. (B) Larval hatching of <i>gfp(RNAi)</i>-treated, <i>Ce-rps-2(RNAi)</i>-treated and <i>Ce-dhc-1(RNAi)</i>-treated PaDAW1 (left y-axes) and <i>C</i>. <i>elegans</i> (right y-axes) 24 h after removal of adults from the plates. Each value represents the mean±s.d. of 6 biological replicates. Conditions where a significant decreases in larval hatching was assessed by multiple comparison of means by Dunnett Contrasts [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0166228#pone.0166228.ref030" target="_blank">30</a>] are indicated with a <i>p</i> value (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0166228#pone.0166228.s003" target="_blank">S2 File</a>).