Figure 1. Design and baseline parameters of a B. pertussis challenge model in protected and naive unprotected mice.

<br>(A) Schematic diagram of animal pre-treatment, sacrifice, sampling and systems analysis on 4 hours and 2, 7, 10 and 14 days p.c. in a <i>B. pertussis </i>challenge model in protected and unprotected BALB/c mice. Pulmonary transcriptomic profile, percentage of splenic Gr1⁺ cells (neutrophils), serum and lung cytokine profiles, serum and lung antibody profiles, and specific splenic CD4⁺CD44⁺ T-cells were assessed at the given time points in protected and unprotected mice. (B) Study parameters at baseline (D0) of naive unprotected mice and protected mice, 56 days after primary infection, including time frames of lung clearance, systemic T-helper subsets, serum IgG profile, mucosal IgA, and pulmonary transcriptomic profile as obtained from data in the current study and data adapted from our previous study<i> </i>(<a>10</a>). 320 genes are differential expressed in protected mice compared to naive (D0 unprotected) mice. (C) An overrepresentation analysis was performed using DAVID for Keywords, KEGG-pathways, and gene ontology biological pathways (GO-BP) to determine the function of the 320 genes. For each term, the number of upregulated (red) and downregulated (green) genes are depicted