Simultaneous expression (and export) of two proteins of the same family in a single blood stage parasite (trophozoites or schizonts) of double-gene tagging (DGT) mutants.
- Aurélie Fougère (3349136)
- Andrew P. Jackson (240893)
- Dafni Paraskevi Bechtsi (3349130)
- Joanna A. M. Braks (15290)
- Takeshi Annoura (164938)
- Jannik Fonager (257212)
- Roberta Spaccapelo (125111)
- Jai Ramesar (49002)
- Séverine Chevalley-Maurel (190439)
- Onny Klop (182503)
- Annelies M. A. van der Laan (260898)
- Hans J. Tanke (260904)
- Clemens H. M. Kocken (219247)
- Erica M. Pasini (3146643)
- Shahid M. Khan (190458)
- Ulrike Böhme (3349133)
- Christiaan van Ooij (48995)
- Thomas D. Otto (188722)
- Chris J. Janse (12383)
- Blandine Franke-Fayard (15291)
Publication date
November 2016
DOIAbstract
<p>These mutants contain the following pairs of genes tagged with either mCherry or GFP: <i>fam-a1/fam-a2</i> (2 independent mutants; panel A, B), <i>fam-b1/fam-b2</i> (panel C<i>) and pir1/pir3</i> (panel D); RMgm ID as indicated in <b><a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005917#ppat.1005917.t002" target="_blank">Table 2</a></b>. Localisation of mCherry-tagged members in the trophozoite stage (upper two rows) and in the schizont (low row) stage. Parasite nuclei are stained with Hoechst, BF bright field. scale bar: 5μm.</p
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