Effect of temperature and pH on full length and truncated EstRM.

<p>(A) Relative activity of ester hydrolysis at different temperatures. (B) Relative activity of ester hydrolysis at different pHs. Full-length EstRM is shown in black, the truncated esterase (ΔEstRM) in grey. 4-nitrophenyl-octanoate (1 mM) was used as a substrate to monitor ester hydrolysis. Relative activities expressed as percentages of maximal activity were plotted for each temperature and pH point. Results are presented as means ± S.D. of triplicate experiments.