Alterations to VEGF-A and TSP-1 with elevated CORT and concomitant prazosin treatment.

<p>RNA or protein was isolated from the TA muscle after 1W or 2W of CORT with or without concurrent prazosin treatment. Taqman qPCR was used to assess the mRNA levels of VEGF-A (A,B) and TSP-1 (D,E), while VEGF-A protein was assessed by ELISA (C) and TSP-1 protein by Western blot (F). (A) VEGF-A mRNA was not altered in response to 1W CORT and/or prazosin. (B) VEGF-A mRNA was not affected by 2W CORT (<i>P</i> = 0.08), while a significant prazosin effect was detected within the 2W CORT-prazosin group (*<i>P</i><0.05). (C) At the 2W time point, VEGF-A protein displayed a tendency for a CORT effect (<i>P</i> = 0.08), and was significantly increased in the CORT-prazosin cohort compared to water CORT animals (*<i>P</i><0.05). (D, E) TSP-1 mRNA was not altered by 1W or 2W CORT and/or prazosin. (F) TSP-1 protein level was significantly reduced with 2W CORT treatment (^#<i>P</i><0.05), and there was a trend (<i>P</i> = 0.09) for an increase in TSP-1 after 2W of prazosin treatment within control animals (n = 4–9).