Treatment of Palmitate induced cell apoptosis dose-dependently.

<p>A: INS-1 cells were incubated with indicated concentration of palmitate for 24 h, cell apoptosis was detected by PI staining. PI-positive cells (red) indicate apoptotic cells and Hoechst-positive cells (blue) indicate live cells. Images shown here are representatives of three independent experiments. Scale bar, 20 μm. B: Quantification of the percentage of PI-positive INS-1 cells treated with palmitate. C: INS-1 cells were incubated with palmitate for 24 h, and expression of cleaved Caspase-3 was detected by western blot. Data shown are means±SEM and representative of three separate experiments. *, p<0.05 compared with control; **, p<0.01 compared with control.