CDC-42 Orients Cell Migration during Epithelial Intercalation in the <i>Caenorhabditis elegans</i> Epidermis - Fig 4

<p>Localization of a CDC-42 biosensor (GFP::WSP-1(<b>G</b>-protein <b>b</b>inding <b>d</b>omain[GBD])) during dorsal intercalation A) in wild-type embryos. Micrographs pseudocolored according to fluorescence intensity using the “Fire” lookup table in ImageJ. As the medial edge becomes pointed, it accumulates active CDC-42 (white arrows), which is maintained during intercalation. Scale bar = 5 μm. B) Comparison of PH::mCherry in wild-type embryos and CDC-42 biosensor localization in <i>par-6</i>::<i>ZF1</i>, <i>vab-1</i>, and otherwise wild-type embryos during dorsal intercalation as in A). Scale bar = 10 μm. C) Quantification of the medial versus lateral enrichment of active CDC-42 in various genotypes. Medial enrichment was significantly greater for GFP::WSP-1(GBD) than PH::mCherry controls (Student’s T-Test, p = 0.01; **). Medial enrichment of GFP::WSP-1(GBD) was significantly decreased in both the <i>par-6</i>::<i>ZF1; par-6(tm1425)</i> and <i>vab-1(dx31)</i> backgrounds (Student’s T-Test, p≤0.01; **). Numbers indicate number of cells analyzed per genotype.