Total erythroid cells in control and AIHA induced mice.

<p>Mice were given <i>i</i>.<i>p</i>. injections of 2x10⁸ rat erythrocytes weekly for 5–6 weeks to induce AIHA. Mice were sacrificed 3 days after 5^th and 6^th doses of injection and their BM and spleen cells were harvested. Cells isolated were stained with anti-mouse CD71-PE, anti-mouse Ter119-APC and 7AAD, after blocking with anti-mouse CD16/32, and the proportions of erythroid cells (as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0166878#pone.0166878.s003" target="_blank">S3 Fig</a>) were determined. Proportions of erythroid cells in BM and spleen of mice are given above (BM <i>p</i><0.001 and spleen <i>p</i> = 0.061, ANOVA). Each value represents mean ± SEM of observations. n = 4 control and 6 AIHA mice. *<i>p</i><0.05, ***<i>p</i><0.005 and ****<i>p</i><0.001 for comparison of the groups (Student t-test).