Additional file 6: Figure S4. of Nitric oxide has contrasting age-dependent effects on the functionality of murine hematopoietic stem cells

Treatment of adult HSCs with NO donor does not induce lineage bias in regenerated blood cells. Lin− cells from adult mice (10–12 weeks; CD45.1)) were treated with 200 μM SNP for 3 days and were infused into irradiated recipients (8–10 weeks; CD45.2). Six mice were used per group. Donor cell chimerism was determined by flow cytometry of recipients’ PB. (a) Experimental scheme is illustrated. (b) Gating strategy used to analyze donor cell chimerism in the PB of recipient mice. (c, d) Lineage commitment in regenerated hematopoietic cells in the PB of recipients at 4 and 16 weeks post-transplant is illustrated. Also see Fig. 7. (TIF 2833 kb