Additional file 4: Figure S3. of Aberrant DNA hypermethylation-silenced SOX21-AS1 gene expression and its clinical importance in oral cancer
- Cheng-Mei Yang (3459206)
- Tsung-Han Wang (3459212)
- Hung-Chih Chen (848200)
- Sung-Chou Li (19928)
- Ming-Chien Lee (3459224)
- Huei-Han Liou (3459209)
- Pei-Feng Liu (3122136)
- Yu-Kai Tseng (3459215)
- Yow-Ling Shiue (3459221)
- Luo-Ping Ger (3459227)
- Kuo-Wang Tsai (3459218)
Publication date
November 2016
DOIAbstract
The cellular localization of SOX21-AS1 and putative mechanism were examined in SAS cell. (A) Cell-fractioned RNA was isolated from the nuclei and cytoplasms of SAS cells. Expression levels of SOX21-AS1 were detected using RT-qPCR. (B) Expression levels of SOX21 were examined using real-time PCR after SOX21-AS1 was transfected into SAS cells. (C) Expression levels of EMT- and cell cycle-relative genes were examined using a Western blot approach in SAS cells with SOX21-AS1 overexpression. (PPT 2411 kb
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