Detection of TSWV levels in infected Money Maker, NahG and BTH-treated NahG plants.

<p>Quantitative ELISA to detect TSWV at 0.5, 1 and 2 wpi. A polyclonal antibody against TSWV (BR-01, Loewe) and an alkaline phosphatase conjugated antibody were used as primary and secondary antibodies, respectively. Immunodetection was carried out by using p-nitrophenyl phosphate as a substrate and the absorbance was measured at 405 nm. Data correspond to the mean ± SD of three biological replicates of one representative experiment.