<i>K</i>. <i>marxianus</i> induces a strong T_reg cell response whereas <i>S</i>. <i>boulardii</i> induces a T cell response comprised of IFNγ, IL-17, and IL-10.

<p>T cell responses following 3 days co-incubation of yeast stimulated DCs and naive autologous T cells. For each yeast, DC stimulation was performed at a yeast:DC ratio of 10:1 (MOI 10), and the subsequent co-incubation of DCs and naive T cells was performed at a DC:T cell ratio of 1:20. <b>A.</b> T cell secretion levels of IFNγ, IL-17, and IL-10. Data are representative of at least five independent experiments, error bars represent SEM. <b>B.</b> Quantification of the T_reg subset as CD4⁺CD25⁺Foxp3⁺ cells. Dotplots gated on the CD4⁺ cell population from a single representative donor displaying the percentage of cells in each quadrant. <b>C.</b> Data from three independent experiments (three donors) expressed as the percentage of CD25⁺Foxp3⁺ cells in the CD4⁺ cell population, error bars represent SEM. One-way ANOVA, Tukey’s multiple comparisons test, indicating significant differences as follows: *, P<0.05; **, P<0.01; ***, P<0.001; ns, not significant.