Additional file 1: of Inhibition of bromodomain and extra-terminal (BET) proteins increases NKG2D ligand MICA expression and sensitivity to NK cell-mediated cytotoxicity in multiple myeloma cells: role of cMYC-IRF4-miR-125b interplay

BETi upregulate MICA expression in different human MM cell lines. MICA, MICB, and PVR/CD155 cell surface expression were analyzed by immunofluorescence and flow cytometry on RPMI-8226, U266, ARP-1, and JJN3, respectively, after a 72 h treatment with JQ1. The MFI of MICA, MICB, and PVR/CD155 was calculated based on at least four independent experiments and evaluated by paired Student t test (*P < 0.05). In the insert, a representative histogram of MICA upregulation is shown. The grey-colored histograms represent basal expression of the indicated ligand, while thick black histograms represent the expression after treatment with the drug. (TIF 3542 kb