Photographs of various stages of the new method.

<p>a, b. The mouse was located on its right side and connected with ventilation and a Data Analysis System. The skin was depilated and disinfected. c. The skin was covered with aseptic hole-towel. d. A 1 cm skin incision and a purse suture were made. e. The pectoralis muscles were separated and the intercostal muscles were exhibited. f. A small hole was made at the fourth intercostal muscle. g. A small wet sterile gauze was used to protect the lungs. The LAD (↑) and left auricle (◀) were displayed. h. One hand held the microneedle holder with a surgical suture needle to pass underneath the LAD and another hand held a smooth tying forceps to widen the hole. i. An approximate 2-mm diameter loop was made. j. A 4–0 suture was passed through the third and fifth intercostal muscle. k. A deflated balloon was placed in the loop. l. The balloon was inflated. m. The chest cavity was closed by straining the purse suture. n. The wound was closed. o. In the classic method, a PE-10 tube was used and a slipknot was made.