Optimized Jasmonic Acid Production by <i>Lasiodiplodia theobromae</i> Reveals Formation of Valuable Plant Secondary Metabolites - Fig 2

<p>Time-course of biomass formation (<b>A</b>), glucose consumption (<b>B</b>), pH-changes (<b>C</b>), OPC-4-formation (<b>D</b>), JA-formation (<b>E</b>) and 11/12-hydroxy-JA formation (<b>F</b>) by <i>L</i>. <i>theobromae</i> strain 2334. Cultures were grown in 100 mL (blue line), 250 mL (red line) and 500 mL (green line) flasks with KNO₃ as nitrogen source. Additionally, cultures were cultivated in 250 mL flasks containing medium supplemented with NH₄NO₃ as nitrogen source (violet line). Data are mean of 2–4 replicates. Two replicates: Red– 1.5 d, green– 1.5/3/6 d, violet 1.5 d and the glucose consumption. Four replicates: Red/green/violet– 12 d. The other data represent three replicates. Abbreviations: JA, jasmonic acid; OPC-4, 3-oxo-2-(pent-2-enyl)-cyclopentane-1-butanoic acid.