Differentiation potential of bovine-induced trophoblastic cells (biTBCs).

<p>(A) Phase-contrast image of biTBCs cultured in the absence of Dox (- Dox) for 7 days. The circled dotted area indicates the colony. (B) Phase-contrast image of biTBCs cultured in the absence of Dox and in the presence of FGF4 and heparin for 7 days. The circled dotted area indicates the colony. (C) Phase-contrast image of TVs generated from biTBCs. (D) Phase-contrast image of differentiated biTBCs. Nuclei were stained with Hoechst. Arrowheads indicate binucleated cells. (E) Endogenous and exogenous gene expression in differentiated biTBCs. iTB#2: Induced trophoblast cell line #2; iTB#2 TV: TVs generated from biTBC line #2 by culturing in low-adhesion culture dishes for 6 days; iTB#2 dif, biTBC line #2 after culturing in the absence of Dox for over 30 days. (A)-(C) scale bars = 500 μm. (D) scale bars = 70 μm.