MIito-ID^® Red probe was used to detect the distribution of mitochondria induced by <i>Brucella melitensis</i> 16M.

<p>The interference groups of I-A-LC3 cells, overexpression group of O-A-LC3 cells, overexpression-interference group of OA-IA-LC3 cells, and the normal group of RAW264.7 cells were passaged and seeded into confocal dishes. Both untreated and NAC-pretreated groups were set up and infected by <i>Brucella melitensis</i> 16M. At 6 and 12 h after infection, mitochondria distribution and GFP-LC3 expression were examined under a confocal laser microscope.