Homodimeric features.

<p>(<i>a</i>) Estimation of the molecular weight. TON_0340 (100 μM) in Buffer A was loaded on a Superdex 75 10/300 GL analytical column, and eluted at a rate of 0.5 ml/min. The elution profile is shown together with those of the size marker proteins, which were conalbumin (75 kDa), ovalbumin (44 kDa), carbonic anhydrase (29 kDa), ribonuclease A (14 kDa), aprotinin (6.5 kDa).(<i>b</i>) Two different homodimeric arrangements in the crystals. Shown are the two TON_0340 molecules forming the crystallographic homodimer in the crystals of Mn²⁺-bound TON_0340 or apo TON_0340. The arrows indicate the relative orientations of the molecules. The circle on the bottom left panel indicates the metal-free clefts.