Result of multiplex PCR detection for dead and viable pathogens via PMA treatment.

<p>Lane M shows the 1000 bp DNA marker. Lanes 1–4 show genomic DNA extracted from dead <i>L</i>. <i>monocytogenes</i>, <i>E</i>. <i>coli</i> O157:H7, and <i>S</i>. <i>aureus</i> following PMA treatment (5 μg/ml, 10 μg/ml, 20 μg/ml, and 40 μg/ml, respectively). Lanes 5–8 show genomic DNA extracted from viable <i>L</i>. <i>monocytogenes</i>, <i>E</i>. <i>coli</i> O157:H7, and <i>S</i>. <i>aureus</i> after PMA treatment (5 μg/ml, 10 μg/ml, 20 μg/ml, and 40 μg/ml).