Disruption of the Ank2L exon compromises the axonal diffusion barrier.

<p>A. Example images of dendrite and axon bleaching experiments in <i>Ank2</i>^{<i>f02001</i>} homozygous animals are shown. The fluorescent marker is mCD8-RFP as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006457#pgen.1006457.g007" target="_blank">Fig 7</a>. B. Quantitation of FRAP experiments is shown. The control data is the 3-day dataset shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006457#pgen.1006457.g007" target="_blank">Fig 7</a>. This is the matched control for the <i>Ank2</i>^{<i>f02001</i>} mutants as the age of the animal, Gal4 driver and fluorescent marker are the same. N’s for the control are listed in the legend for <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006457#pgen.1006457.g007" target="_blank">Fig 7</a>. For the mutant, FRAP was measured in dendrites of 9 cells and axons of 19 cells. C. The recovery plateau was quantitated by averaging values between 110 and 120 ms. Bars show mean ± SEM. and the asterisk indicates significant difference (p < 0.02, t-test).