Effect of Ado on proliferation of MDA-MB-231 cells.

<p>(A) Incorporation of [³H]-thymidine into cells incubated for 48–72 h in the absence (Control) or the presence of Ado (50 μM). (B) Dose dependence of the effects of Ado on the incorporation of [³H]-thymidine. Cells were cultured for 48–72 h in the absence (Control) or presence of various concentrations of Ado. (C) Effect of Ado on cell growth. Cells were plated and after 48or 72 h of incubation, attached cells were trypsinized and counted with the aid of a using a handheld automated cell counter (Scepter 2.0, Millipore).