Clonality analysis of each tumor from patient A1.

<p>VAFs among the primary and matched metastases in patient A1 (A) and a representative evolutionary tree (B) colored by subclone based on the clonality plots in panel A, with the width of the branch indicating the approximate percentage of that clone within the tumor. Clone 1 is established in the primary tumor and seeded all distant metastases. Clones 2 and 4 from the primary tumor seeded the liver and the adrenal gland, with clone 7 concurrently seeding the liver from the primary tumor. Clones 3 and 5 from the primary tumor seeded the lung, with clone 3 also seeding the spine. Private clones include clone 6, specific to the adrenal metastasis; clone 8, specific to the primary tumor; and clone 9, specific to the spinal metastasis.