Activation of CD4⁺ T cells and induction of Treg is similar in FV-infected and co-infected mice.

<p>C57BL/6 mice were left naïve or infected with FV or <i>L</i>. <i>sigmodontis</i> or co-infected with <i>L</i>. <i>sigmodontis</i> and FV. LN and spleen cells were isolated at day 20 p.i. and analysed by flow cytometry. A) Expression of CD43 in CD4⁺ T cells in LN and spleen. Frequencies of Treg in LN (B) and spleen (D) from naïve, <i>L</i>. <i>sigmodontis</i>-infected, FV-infected and co-infected mice. Absolute numbers of Treg in FV-infected and co-infected mice in LN (C) and spleen (E). Data are combined from 2–4 experiments (n = 2–5 mice per group and experiment). Each data point represents an individual mouse. The line shows the mean and statistical significances are indicated between the groups (*p ≤ 0.05, **p ≤ 0.01).