Eya protein is detected in non-transformed <i>so</i>^<i>3</i> mutant clones.

<p>(A-L) Eye discs and adult heads of the genotype: <i>eyflp; FRT42D so</i>^<i>3</i> <i>/FRT42D Ubi-GFP</i> that contain <i>so</i>^<i>3</i> mutant clones. (A-H) Light microscope images of developing eye-antennal discs. Yellow arrows identify <i>so</i>^<i>3</i> clones that have not transformed into head epidermis and still express <i>eya</i>. Green arrows demarcate large <i>so</i>^<i>3</i> clones that are transforming into head epidermis and lack Eya protein. Red = F-actin, green = GFP, blue = Eya. (I-L) SEM images of adult <i>Drosophila</i> compound eyes and heads. Green arrows indicate regions of the compound eye that has transformed into head epidermis. Green arrows in panels I-L indicated regions of head epidermis bifurcating the retinal field. Anterior is to the right in all adult head and imaginal disc images. At least 30 adult eyes and imaginal discs containing <i>so</i>^<i>3</i> clones were examined. Scale bar, 100μm.