Impact of CCR2 deficiency in resident cells of the CNS or in the hematopoietic system on cytokines and chemokines production during HSE.

<p>Levels of cytokines and chemokines in brain homogenates of wild-type (WT) C57BL/6, CCR2^-/-, CCR2^-/-→WT and WT→CCR2^-/- mice were evaluated prior to (day 0) and on days 6, 8 and 10 following intranasal infection with 1.2x10⁶ PFU of HSV-1. Levels of cytokines and chemokines were measured using magnetic bead-based immunoassay. Results are expressed in pg/mL of brain homogenates and represent the means ± standard deviations of 4 to 5 mice per group at each time point. Statistical analyses were performed using a one-way analysis of variance (ANOVA) with Tukey’s multiple comparison post-test. Statistically significant results obtained in CCR2^-/-, CCR2^-/-→WT and WT→CCR2^-/- compared to those of the WT group are indicated as follows: *, <i>P</i><0.05; **, <i>P</i><0.01; ***, <i>P</i><0.001.