miR-151-3p reduces TWIST1 mRNA and protein expression in human breast cancer cells.

<p>(A) After infection with adenoviruses expressing miR-151 (Ad-miR-151) or GFP (Ad-GFP) for 48 hours, the TWIST1 mRNA expression levels of MDA-MB-231 cells were determined using quantitative real-time PCR. The data were normalized to GAPDH mRNA levels. (B) After infection with adenoviruses expressing miR-151 (Ad-miR-151) or GFP (Ad-GFP) for 48 hours, the TWIST1 protein expression levels of MDA-MB-231 cells were determined using Western blotting. The data were normalized to α-tubulin protein levels. All experiments were performed in triplicate. Each bar represents the mean ± SEM (standard error of the mean). *, <i>P</i> < 0.05. (C) miR-151 and TWIST1 expression levels of breast cancer samples (N = 648) were downloaded from the “The Cancer Genome Atlas” (TCGA) and the Broad GDAC Firehose data portal. The correlation between miR-151 and FAK expression levels was analyzed. The results shown here are reported in their entirety based upon data generated by the TCGA Research Network: <a href="http://cancergenome.nih.gov" target="_blank">http://cancergenome.nih.gov</a>.