Architecture and cellular structure of WT spleen and WT into LTβR^-/- splenic regenerates are comparable.

<p>(A) The compartment sizes of normal WT spleens were analyzed and compared to that of splenic regenerates (WT→LTβR^-/-) 8 weeks after implantation. Indicated is the size of individual splenic zones as percent of total section area (n = 6–7 animals per group; RP: red pulp, MZ: marginal zone, WP: white pulp, T: T-cell zone, B: B-cell zone, T/B: mixed T/B-cell zone). (B) Frequency of leukocyte subsets in normal WT spleens and splenic regenerates (WT→LTβR^-/-) as determined by flow cytometry (n = 5–8 animals per group; B: B cells, IgD^- B: memory B cells, T: T cells; CD8: CD8⁺ T cells, CD4: CD4⁺ T cells, CD25: CD25⁺CD4⁺ -regulatory- T cells). (C) Expression of cytokines and chemokines in normal WT spleens and splenic regenerates (WT→ LTβR^-/-). Shown is the relative expression level normalized to the housekeeping gene (MLN 51) and relative to WT (fold expression change). The bars represent the mean values ± SD. Mann-Whitney <i>U</i> test was used to indicate a significant difference between WT spleens and splenic regenerates (*p < 0.05; ***p < 0.001).