Novel Methods to Generate Active Ingredients-Enriched Ashwagandha Leaves and Extracts - Fig 1

<p>(<b>A</b>) HPLC analyses of alcoholic extracts of Ashwagandha roots (1) and leaves (2) raised at distant places, Ibaraki (K) and Tokushima (A and B) and Punjab, India (P). Purified Withaferin A (Wi-A) and Withanone (Wi-N) were used as standards. (<b>B</b>) Withanolide yield and ratio of Wi-N/Wi-A in extracts from root and leaves of Ashwagandha grown at distant places are shown. (<b>C</b>) Representative comparative cytotoxicity assay for root and leaf extracts of Ashwagandha. Cytotoxicity as determined by viability assay (short term cytotoxicity) and phase contrast images of cells in plates for colony forming assays (long term cytotoxicity) are shown.