Flow cytometry (FCM) profiles.

<p>(A) Diploid <i>B</i>. <i>distachyon</i> Bd21 and (B) diploid <i>B</i>. <i>stacei</i> ABR114 showing two peaks corresponding to the G1 (2C DNA) and G2/M (4C DNA) nuclei. (C) The F1 interspecific hybrid of <i>B</i>. <i>distachyon</i> Bd21 and <i>B</i>. <i>stacei</i> ABR114, with a profile similar to that of the parental species. (D) Position of the 2C and 4C peaks for <i>B</i>. <i>hybridum</i> ABR113 indicate a nuclear genome size twice that of <i>B</i>. <i>distachyon</i> or <i>B</i>. <i>stacei</i>. (E) FCM profile of the synthetic allopolyploid allo21×114 (S0 generation) is similar to that of natural <i>B</i>. <i>hybridum</i> (D) and double the genome size of the F1 (C) and diploid parental species (A-B). The X and Y axes show relative DNA content per nuclei estimated by fluorescence intensity and the frequency-count (cell number), respectively.