Ectopic miR-182 expression induces RPE cell G1 arrest and inhibits cell proliferation.

<p>(A) MTS cell proliferation assay was carried out as indicated after lipofectamine transfection of RPE cells with either miR-182 (50 nM) or a negative control (NC). The data are expressed as the mean value ± SEM of the results obtained from triplicates in one experiment. Results represent those obtained in three separate experiments. (B) RPE cells were collected 48 hours after transfection with miR-182 or NC, stained with propidium iodide and analyzed by flow cytometry. Ten thousand cells were evaluated in each sample. The most representative results in three independent experiments are depicted. (C) miR-182 regulated cell cycle-related proteins that are important for cell cycle G1 phase progression and G1/S transition. RPE cells were transfected with miR-182, or a negative control. Cell lysates were prepared and used for Western blot analysis with phosphorylated Rb (p-Rb) antibody. GAPDH was used as a loading control. ***: Differences in cell proliferation between miR-182 and NC transfected cells were significant, <i>P</i> < 0.001.