Histological analysis showed that CBP inhibited neutrophil influx in an inflammatory skin model.

<p>Twelve mice were injected in the dermis with 1 μg LPS with and without various amounts of CBP. In addition each mouse received injections of CBP only and PBS. i.e. a total of four injections per mouse (n = 4 animals per group for each dose of CBP). Mice were sacrificed at 12 h post treatment and skin samples were fixed in zinc salts solution and paraffin-embedded. (A) Skin sections were stained for neutrophils with triple immunofluorescent antibodies (AF594-GR1, FITC-MHC-II and DAPI) and visualised by fluorescent microscopy with 20x magnification. Scale bar = 20 μm. N = normal skin, P = PBS, L = LPS, L+C = LPS + CBP, C = CBP, iso = isotype control. (B) Numbers of Gr-1⁺ MHC-II⁺ neutrophils in four fields of sections from three zones with 50 μm intervals were enumerated. The data shown are the mean ± SD and the significant difference is indicated by the asterisk (<i>P</i> < 0.05, Tukey’s test ANOVA, GraphPad Prism).