Additional file 3: Figure S3. of Attenuation of chemokine receptor function and surface expression as an immunomodulatory strategy employed by human cytomegalovirus is linked to vGPCR US28

β-arrestin 2 pool titrations. HEK293T cells were cotransfected with fixed amounts of CXCR4-ElucC and mock (a) or US28wt (b) cDNA and the DNA amount for ElucN-β-arrestin 2 was increased. After stimulation with vehicle or 100 nM CXCL12, luminescence was measured. ΔLuminesence was calculated by subtracting luminescence detected in vehicle stimulated cells from luminescence detected in cells stimulated with ligand for each transfection. Columns represent means ± SEM of at least two independent experiments each performed in quadruplicates. (TIF 130557 kb