Growth curves of wild type, <i>cut3</i> Val223Ala and <i>plcA</i> His39Arg mutant strains in liquid medium, THP-1 cells and murine lung.

<p><b>(A and C) Growth curves in liquid medium</b>. Growth curves in liquid medium over 16 days of incubation for <i>cut3</i> Val223Ala mutant (<b>A</b>) and <i>plcA</i> His39Arg mutant (<b>C</b>) strains. <b>(B and D) Growth curves in THP-1 cells</b>. Growth curves of <i>cut3</i> Val223Ala mutant <b>(B)</b> and <i>plcA</i> His39Arg mutant (<b>D</b>) strains in THP-1 cells over 6 days of infection. Data are expressed as CFU increase relative to day 0. <b>(E) Growth curves in murine lung.</b> Growth curves of wild type, <i>cut3</i> Val223Ala mutant, and <i>plcA</i> His39Arg mutant strains in the murine lung up to 12 weeks post-infection. Panels A-D show the mean and standard deviation of results obtained from three replicates; panel E shows the mean and standard deviation of results obtained from 3–4 animals per time point.