Regulation of the Human Telomerase Gene <i>TERT</i> by Telomere Position Effect—Over Long Distances (TPE-OLD): Implications for Aging and Cancer

<div><p>Telomerase is expressed in early human development and then becomes silenced in most normal tissues. Because ~90% of primary human tumors express telomerase and generally maintain very short telomeres, telomerase is carefully regulated, particularly in large, long-lived mammals. In the current report, we provide substantial evidence for a new regulatory control mechanism of the rate limiting catalytic protein component of telomerase (h<i>TERT</i>) that is determined by the length of telomeres. We document that normal, young human cells with long telomeres have a repressed h<i>TERT</i> epigenetic status (chromatin and DNA methylation), but the epigenetic status is altered when telomeres become short. The change in epigenetic status correlates with altered expression of <i>TERT</i> and genes near to <i>TERT</i>, indicating a change in chromatin. Furthermore, we identified a chromosome 5p telomere loop to a region near <i>TERT</i> in human cells with long telomeres that is disengaged with increased cell divisions as telomeres progressively shorten. Finally, we provide support for a role of the TRF2 protein, and possibly TERRA, in the telomere looping maintenance mechanism through interactions with interstitial TTAGGG repeats. This provides new insights into how the changes in genome structure during replicative aging result in an increased susceptibility to age-related diseases and cancer prior to the initiation of a DNA damage signal.</p>