The deletion of <i>Hotair</i> affects the expression of the neighboring <i>Hoxc11</i> and <i>Hoxc12</i> genes.

<p><i>Hoxc12</i> (A) and <i>Hoxc11</i> (B) expression (normalized RPKM values) in the various dissected tissue samples for wild type (green) and <i>Del(Hotair)</i>^<i>-/-</i> (orange) E12.5 embryos. The asterisk* indicates those samples where significant differences in transcript levels between genotypes were scored (FDR < 10%). (C) WISH using the <i>Hoxc12</i> probe in both wild type (left) and <i>Del(Hotair)</i>^<i>-/-</i> (right) E12.5 embryos. The spatial expression of <i>Hoxc12</i> remains globally unchanged. D) WISH of <i>Hoxc11</i> in wild type (left) and <i>Del(Hotair)</i>^<i>-/-</i> (right) E12.5 embryos. The arrows indicate the slight anterior shift in the expression profile and the increase in signal intensity in the mutant genotype.