Loss of RMI2 causes significant elevation in UFBs.

<p>Representative images and RMI2 wild-type and null HCT-116 cells stained with anti-PICH (red) and DAPI for DNA (blue) for anaphase A (A) and anaphase B (B). Scale bar 5 μm. Quantification of PICH fiber detection in anaphase A cells in (C) and anaphase B (D) from wild-type HCT-116 control and RIM2 null cells (1–2, 1–3, 4–6). Data taken from three independent experiments, with 20 anaphase A and 20 anaphase B cells scored for each HCT-116 cell line (wild-type, 1–2, 1–3, 4–6) per experiment. Error bars represent standard error of the mean.