Developing a cellular model of FcγRIa function.

<p>(A) Diagram illustrating the phagocytic assay used to reconstitute FcγR function using Alexa Fluor 488 IgG (green) and DyLight 405 (blue) IgG-labeled polystyrene beads. (B) Representative fluorescence microscopy images of U-2 OS cells transduced with lentivirus co-expressing TagRFP and Fluc, FcγRIa, or FcγRIIa, incubated with Alexa Fluor 488 IgG-opsonized beads (green) for 1.5 h, followed by secondary DyLight 405 IgG labeling (blue) of external beads. (C) Quantification of phagocytosed IgG-coated beads. Error bars represent s.d., 160 cells were counted for each of three independent experiments.