Evolution of physiological differentiation parameters.

<p>(A) T2EC were induced to differentiate for 24 and 48 h and subsequently seeded back in self-renewal conditions. Cells were then counted every day for 5 d. The green curve represents the growth of cells induced to differentiate for 24 h and the orange curve indicates the growth of cells induced to differentiate for 48 h. The data shown are the mean +/− standard deviation calculated on the basis of three independent experiments for the time-points 72 h and 96 h and four experiments for all other time-points. The growth ratio was computed as the cell number divided by the total cells at day 0. The significance of the difference between growth ratios at 24 h and 48 h was calculated using a Wilcoxon test. (B) The boxplots of the mean size observed were based on four independent experiments, each using 50,000 cells, using FSC_A as a proxy for cell size. All of the variances were compared by pairs using the F test and the * indicates when the variances were significantly different. Data for this figure can be found at osf.io/k2q5b.