Identification and mapping of complete set of ribose and base modifications of 18S rRNA.

<p>Analysis of the composition of 45 fragments derived from 18S rRNA using MBN assay permitted identification of all ribose and base modifications of 18S rRNA. A) Overlaid chromatograms of different fragments identified to contain Am residues; (B) Gm residues; (C) Cm residues; (D) Um residues; (E) m⁷G, and (F) ac⁴C residues. Location of each modification along with the oligonucleotide number used for the isolation of respective fragment are mentioned on the right side of the peak.