MOESM11 of Nannochloropsis, a rich source of diacylglycerol acyltransferases for engineering of triacylglycerol content in different hosts

Additional file 11: Figure S10. (A–B) Relative expression of NoDGTT5 analyzed by qPCR in the leaves (A) and seeds (B) of Arabidopsis lines transformed with 35S:NoDGTT5 construct. (C) Analysis for the insertion of T-DNA by PCR using genomic DNA from the wild-type plants (AtWT), control lines (AtEV) and two tag1-1 lines (tag1-1a and tag1-1b). (D) Relative expression of NoDGTT5 analyzed by qPCR in the seeds of two tag1-1 lines complemented with NoDGTT5. In A and B arrowheads indicate the lines exhibiting the highest NoDGTT5 expression and chosen for lipid and microscopic analyses. Each data point represents the average of three biological replicates. Each sample was analyzed in technical triplicates and normalized to actin as a reference gene. Values represent means ± SD (n = 3), with asterisks indicating P < 0.01