Evidence That Trimethyllysine Hydroxylase Catalyzes the Formation of (2<i>S</i>,3<i>S</i>)‑3-Hydroxy‑<i>N</i>^ε‑trimethyllysine

Trimethyllysine hydroxylase (TMLH) is an Fe­(II) and 2-oxoglutarate (2OG) dependent oxygenase involved in the biomedically important carnitine biosynthesis pathway. A combination of synthetic and NMR studies provides direct evidence that human TMLH catalyzes the stereoselective conversion of (2<i>S</i>)-<i>N</i>^ε-trimethyllysine to (2<i>S</i>,3<i>S</i>)-3-hydroxy-<i>N</i>^ε-trimethyllysine