<p>Bacteria in logarithmic growth were exposed to 32 μg/ml PlySs2 for 30 minutes in PB (for 60-minute readings, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0169180#pone.0169180.s004" target="_blank">S3 Fig</a>). The activity was measured by OD<sub>600</sub> reduction. To normalize and combine values from multiple tests, the final OD<sub>600</sub> of the treated samples was divided by the final OD<sub>600</sub> of the untreated samples. An OD<sub>600</sub> ratio of 1.0 indicates no lysis, while an OD<sub>600</sub> ratio of ~0.02 indicates complete lysis.</p
<p>Initial ratios were 1∶10<sup>4</sup>, 1∶10<sup>−2</sup>, 1∶1, and 10<sup>4</sup>∶1. Lysogenic cel...
Large circles indicate the absence of lysis; small dots indicate complete lysis right from the begin...
<p>Ratios were determined by comparison of viable counts of released bacteria to the inoculation dos...
<p>(A) <i>S. typhimurium</i> carrying pLYS were grown in LB supplemented with L-arabinose. When the ...
<p>A) 10 µL of bacterial culture of GBS WT, PilB<sup>−</sup> and PilA/C<sup>−</sup> were stained wit...
<p>A549 cells were infected with <i>A</i>. <i>baumannii</i> wild-type or mutant cells for 5 hours. T...
<p>Hemolytic activity of Ply was measured in the presence of 2% RBCs. The results are shown as relat...
<p>PlyPl23 activity on <i>P</i>. <i>larvae</i> Pl02-23, at different enzyme (0.2 μm and 2.0 μm) and ...
<p>Bacteria (<i>E. coli</i> ML-35p and <i>S. aureus</i> ST1065, ∼2–3 10<sup>6</sup> cfu/mL) were dil...
<p>Bacterial strains show variable viability after treatment with PAXgene Fix, PAXgene Fix and Stab ...
<p>For the time-kill experiments (A), logarithmic-phase growing <i>E</i>. <i>coli</i> K12 cells were...
<p>PlyPl23 activity on <i>P</i>. <i>larvae</i> Pl02-23 at different pH values (pH 5.0, 6.0, and 7.0)...
<p>The total number of cells (, ), inhibitors (, ) and binding sites () are plotted in each case. Si...
<p>Effects of <i>potD</i> deletion on hemolytic activity were assessed via hemolysis assay, in which...
<p>A) Lethality analysis as described in the legend of <a href="http://www.plosone.org/article/info:...
<p>Initial ratios were 1∶10<sup>4</sup>, 1∶10<sup>−2</sup>, 1∶1, and 10<sup>4</sup>∶1. Lysogenic cel...
Large circles indicate the absence of lysis; small dots indicate complete lysis right from the begin...
<p>Ratios were determined by comparison of viable counts of released bacteria to the inoculation dos...
<p>(A) <i>S. typhimurium</i> carrying pLYS were grown in LB supplemented with L-arabinose. When the ...
<p>A) 10 µL of bacterial culture of GBS WT, PilB<sup>−</sup> and PilA/C<sup>−</sup> were stained wit...
<p>A549 cells were infected with <i>A</i>. <i>baumannii</i> wild-type or mutant cells for 5 hours. T...
<p>Hemolytic activity of Ply was measured in the presence of 2% RBCs. The results are shown as relat...
<p>PlyPl23 activity on <i>P</i>. <i>larvae</i> Pl02-23, at different enzyme (0.2 μm and 2.0 μm) and ...
<p>Bacteria (<i>E. coli</i> ML-35p and <i>S. aureus</i> ST1065, ∼2–3 10<sup>6</sup> cfu/mL) were dil...
<p>Bacterial strains show variable viability after treatment with PAXgene Fix, PAXgene Fix and Stab ...
<p>For the time-kill experiments (A), logarithmic-phase growing <i>E</i>. <i>coli</i> K12 cells were...
<p>PlyPl23 activity on <i>P</i>. <i>larvae</i> Pl02-23 at different pH values (pH 5.0, 6.0, and 7.0)...
<p>The total number of cells (, ), inhibitors (, ) and binding sites () are plotted in each case. Si...
<p>Effects of <i>potD</i> deletion on hemolytic activity were assessed via hemolysis assay, in which...
<p>A) Lethality analysis as described in the legend of <a href="http://www.plosone.org/article/info:...
<p>Initial ratios were 1∶10<sup>4</sup>, 1∶10<sup>−2</sup>, 1∶1, and 10<sup>4</sup>∶1. Lysogenic cel...
Large circles indicate the absence of lysis; small dots indicate complete lysis right from the begin...
<p>Ratios were determined by comparison of viable counts of released bacteria to the inoculation dos...