Effect of thiol-containing compounds and inorganic compounds on the inhibition of urease activity by Pal.

<p>Thiol reagents protected against HPU (<b>A</b>) and JBU (<b>B</b>) inactivation by Pal. Inorganic compounds protected against HPU (<b>C</b>) and JBU (<b>D</b>) inhibition by Pal. The concentrations of both ureases were 0.25 mg/mL. Concentrations of all thiol-containing compounds were 1.25 mM and the competitive inhibitors were 5 mM. The concentration of Pal was 0.5 mM for both HPU and JBU in the assays. After 5, 10, 20, and 40 min of incubation, residual urease activity was assayed respectively. Residual activity values were expressed as percent of the control activity. The residual activity of urease in the presence of Pal without the protector or urease in the presence of protector without the Pal was given for comparison. Results presented here are the mean value of n = 3 ± SD. *<i>P</i> < 0.05 and **<i>P</i> < 0.01 were considered as significant differences when compared with control (the first column of each group), ^##<i>P</i> < 0.01 in comparison to the control (urease in the presence of protector without the Pal group), and ^Δ<i>P</i> < 0.05 and ^ΔΔ<i>P</i> < 0.01 in comparison to the control (urease in the presence of Pal without the protector), as determined by one-way ANOVA.