Accumulation of GAGs was stained by alcian blue.

<p><b>(A-D)</b> Photographs of monolayer expanded cells cultured for 2 weeks. Scale bar = 10 μm. <b>(E-H)</b> Pellets culture at 4 weeks after differentiation. Scale bar = 20 μm. <b>(I)</b> Morphology of pellet culture at 4 weeks after differentiation.