Axon labeling of neostriatal neurons with the AAV-SynTetOff vectors.

<p>(<b>A</b>) Construction of the vector plasmids, pAAV2-SynTetOff-GFP, pAAV2-SynTetOff-palGFP, pAAV2-SynTetOff-myrGFP, and pAAV2-SynTetOff-FGL. (<b>B–E</b>) Sagittal views of brain sections with injections of the AAV vectors. By addition of the palmitoylation site of the GAP-43 N-terminus (palGFP; C) or the myristoylation/palmitoylation site of the Fyn N-terminus (myrGFP; D), axon fibers in the GPe and SNr were more clearly visualized than with GFP without membrane-targeting signal (B). On the other hand, when a somatodendritic-targeting signal, LDLRct, was added to the C-terminus of myrGFP (FGL; E), axon fiber labeling in the GPe and SNr was mostly suppressed (E₄, E₅). Scale bar in B₁ applies to B₁–E₁, B₂–E₂, and B₃–E₃. Scale bar in B₄ applies to B₄–E₄ and B₅–E₅.