Effect of CIL-102 on cell viability and morphological characteristics of human colorectal cancer DLD-1 cells, and its role in assessing cell death.

<p>(A) DLD-1, HAT-116 and HCoEpiC cell were treated with either 0.1% DMSO (as control) or CIL-102 (0.1–10 mM) for 24 h and the proportion of surviving cells was measured by the MTT assay. (B) Changes in nuclei by DAPI staining. DLD-1 cells were treated with vehicle or 1 mM CIL-102 for 24 h, and stained with DAPI. Apoptotic cells were measured under fluorescence microscopy. The data were presented as the mean of three repeats from one independent experiment. Other data in this figure is presented as mean ± SD of three independent experiments. * indicate means that are significantly different when compared to the control group of DLD-1 with P < 0.05.