Overproduction of α-Lipoic Acid by Gene Manipulated <i>Escherichia coli</i> - Fig 5

<p>(A) Growth of TM179 in E minimal medium containing 1 μL of purified LA from YS56 (a), 1 μL of the purified LA from BL21 (b), 1 ng/mL of standard LA (c), or without any addition of LA (d). (B) The determination of LA concentration by HPLC. Ten microliters of the extracts from YS56 (a) and BL21 (b) were applied. Red lines were obtained with 7.5 μg of standard LA.